Specifica scientists, Frank Erasmus, Sara D’Angelo, Fortunato Ferrara, Leslie Naranjo, and Andrew Bradbury along with New Mexico Consortium scientist, André A. Teixeira have recently put out a new publication on antibody library diversity in a paper titled, A single donor is sufficient to produce a highly functional in vitro antibody library, in Communications Biology.
The most successful class of therapeutics are the monoclonal antibodies. These are now seven of the top ten best selling pharmaceuticals. So, it is extremely important for antibody libraries to be as diverse as possible. This gives a greater probability of pharmaceutical researchers finding what they need to create successful therapeutics.
Up until now the main method of increasing the diversity of an antibody library has been to increase the number of donors.
In this study, the researchers look at taking a different approach. They study the possibility of creating an in vitro antibody library from a single healthy individual. They do this to show that the number of lymphocytes is the major criteria in the production of a diverse and functional antibody library, and not necessarily the number of donors.
This article describes the construction of a high quality library made up of antibodies of a single donor. Each step of the process was carefully followed and validated to ensure quality and diversity. This library was tested and it indeed had the desired biophysical properties.
To learn more see the entire paper at: A single donor is sufficient to produce a highly functional in vitro antibody library.
The above image taken from the article shows a Circos graph of biophysical characteristics in the final library.
Article by Carrie Talus, NMC.